getClusters报错、K-mean聚类用visCluster画线图出错

[EdmundFieldQIN]

matrix <- read.delim("盐胁迫.txt",row.names = 1,header = T)
class(matrix)
#[1] "data.frame"

getClusters(exp = matrix)
错误于sequence.default(n..1, from = seq.int(s.1, length(df), s.1), :
'from' contains NAs
此外: 警告信息:
In sequence.default(n..1, from = seq.int(s.1, length(df), s.1), :
强制到整数范围引入的 NA

ck <- clusterData(exp = matrix,
                    cluster.method = "kmeans",
                    cluster.num = 15)
visCluster(object = ck,
           plot.type = "line")

#> visCluster(object = ck,
#+ plot.type = "line")
#Error in dplyr::arrange():
#ℹ In argument: ..2 = .data[["membership"]].
#Caused by error in .data[["membership"]]:
#! Column membership not found in .data.
#Run rlang::last_trace() to see where the error occurred.

请junjun大神解决一下，感谢

[junjunlab]

检查矩阵是否有NA，有的话替换成0

[elegantmedstu]

https://github.com/cole-trapnell-lab/monocle-release/issues/518。目前似乎没有很好的解决办法，在我的数据中检查了没有NA值，但仍有报错。我的数据表格来自单细胞数据的AverageExpression，这是可能的原因